The new SLENDR technique: Protein labeling in the developing brain by genome editing
Confocal microscopic image of the hippocampus showing immunoreactivities for mEGFP (magenta) and the HA tag (green) fused to β-Actin. Credit: Max Planck Florida Institute for Neuroscience Dr. Ryohei Yasuda and his team have developed a method, called SLENDR, that allows precise modification of neuronal DNA in living samples. Using their new technique, the research team were able to reliably label two different proteins with distinct colors at the same time in the same cell. The researchers used a variety of imaging methods as well as DNA sequencing to confirm that the SLENDR method had truly and precisely knocked in the genes. Ryohei Yasuda, Ph.D. and his team at the Max Planck Florida Institute of Neuroscience (MPFI) are working to understand the way cells in our brains change as we learn…
Link to Full Article: The new SLENDR technique: Protein labeling in the developing brain by genome editing
The new SLENDR technique: Protein labeling in the developing brain by genome editing
